Vascularized axillary lymph node transfer: A novel model in the rat.
Identifieur interne : 001624 ( Main/Exploration ); précédent : 001623; suivant : 001625Vascularized axillary lymph node transfer: A novel model in the rat.
Auteurs : Grzegorz J. Kwiecien [États-Unis] ; Safak Uygur [États-Unis] ; Jason Korn [États-Unis] ; Bahar Bassiri Gharb [États-Unis] ; Maria Madajka [États-Unis] ; Risal Djohan [États-Unis] ; James E. Zins [États-Unis] ; Graham S. Schwarz [États-Unis]Source :
- Microsurgery [ 1098-2752 ] ; 2015.
Descripteurs français
- KwdFr :
- Aine (), Aisselle, Anastomose chirurgicale, Animaux, Artère fémorale (), Lambeaux tissulaires libres (), Lambeaux tissulaires libres (transplantation), Lymphoedème (), Microchirurgie, Modèles animaux, Mâle, Noeuds lymphatiques (), Noeuds lymphatiques (transplantation), Rats, Rats de lignée LEW, Survie du greffon, Veine fémorale (), Études de faisabilité.
- MESH :
English descriptors
- KwdEn :
- Anastomosis, Surgical, Animals, Axilla, Feasibility Studies, Femoral Artery (surgery), Femoral Vein (surgery), Free Tissue Flaps (blood supply), Free Tissue Flaps (transplantation), Graft Survival, Groin (blood supply), Groin (surgery), Lymph Nodes (blood supply), Lymph Nodes (transplantation), Lymphedema (surgery), Male, Microsurgery, Models, Animal, Rats, Rats, Inbred Lew.
- MESH :
- blood supply : Free Tissue Flaps, Groin, Lymph Nodes.
- surgery : Femoral Artery, Femoral Vein, Groin, Lymphedema.
- transplantation : Free Tissue Flaps, Lymph Nodes.
- Anastomosis, Surgical, Animals, Axilla, Feasibility Studies, Graft Survival, Male, Microsurgery, Models, Animal, Rats, Rats, Inbred Lew.
Abstract
Vascularized lymph node transfer (VLNT) is a promising microvascular free flap technique for the surgical treatment of lymphedema. To date, few experimental animal models for VLNT have been described and the viability of lymph nodes after the transfer tested. We aimed to evaluate the feasibility of axillary VLNT in the rat. Lymph node containing flaps were harvested from the axillary region in 10 Lewis rats based on the axillary vessels. Flaps were transferred to the ipsilateral groin and end-to-side microanastomosis was performed to the femoral vessels using 10-0 sutures. Indocyanine green (ICG) angiography was used to confirm flap perfusion. On postoperative day 7, flaps were elevated to assess their structure and vessel patency. Hematoxylin and eosin staining was used to confirm the presence and survival of lymph nodes. All animals tolerated the procedure well. Immediate post-procedure ICG angiography confirmed flap perfusion. No signs of ischemia or necrosis were observed in donor extremities. At postoperative day 7, all flaps remained viable with patent vascular pedicles. Gross examination and histology confirmed the presence of 3.6 ± 0.5 lymph nodes in each flap without any signs of necrosis. This study showed that the transfer of axillary lymph nodes based on the axillary vessels is feasible. The flap can be used without the need for donor animals and it contains a consistent number of lymph nodes. This reliable VLNT can be further utilized in studies involving lymphedema, transplantation, and induction of immunologic tolerance.
DOI: 10.1002/micr.22472
PubMed: 26366994
Affiliations:
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Kwiecien</name><affiliation wicri:level="2"><nlm:affiliation>Department of Plastic Surgery, Cleveland Clinic, Cleveland, OH.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>Department of Plastic Surgery, Cleveland Clinic, Cleveland</wicri:cityArea></affiliation></author><author><name sortKey="Uygur, Safak" sort="Uygur, Safak" uniqKey="Uygur S" first="Safak" last="Uygur">Safak Uygur</name><affiliation wicri:level="2"><nlm:affiliation>Department of Plastic Surgery, Cleveland Clinic, Cleveland, OH.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>Department of Plastic Surgery, Cleveland Clinic, Cleveland</wicri:cityArea></affiliation></author><author><name sortKey="Korn, Jason" sort="Korn, Jason" uniqKey="Korn J" first="Jason" last="Korn">Jason Korn</name><affiliation wicri:level="2"><nlm:affiliation>Department of Plastic Surgery, Cleveland Clinic, Cleveland, OH.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>Department of Plastic Surgery, Cleveland Clinic, Cleveland</wicri:cityArea></affiliation></author><author><name sortKey="Gharb, Bahar Bassiri" sort="Gharb, Bahar Bassiri" uniqKey="Gharb B" first="Bahar Bassiri" last="Gharb">Bahar Bassiri Gharb</name><affiliation wicri:level="2"><nlm:affiliation>Department of Plastic Surgery, Cleveland Clinic, Cleveland, OH.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>Department of Plastic Surgery, Cleveland Clinic, Cleveland</wicri:cityArea></affiliation></author><author><name sortKey="Madajka, Maria" sort="Madajka, Maria" uniqKey="Madajka M" first="Maria" last="Madajka">Maria Madajka</name><affiliation wicri:level="2"><nlm:affiliation>Department of Plastic Surgery, Cleveland Clinic, Cleveland, OH.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>Department of Plastic Surgery, Cleveland Clinic, Cleveland</wicri:cityArea></affiliation></author><author><name sortKey="Djohan, Risal" sort="Djohan, Risal" uniqKey="Djohan R" first="Risal" last="Djohan">Risal Djohan</name><affiliation wicri:level="2"><nlm:affiliation>Department of Plastic Surgery, Cleveland Clinic, Cleveland, OH.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>Department of Plastic Surgery, Cleveland Clinic, Cleveland</wicri:cityArea></affiliation></author><author><name sortKey="Zins, James E" sort="Zins, James E" uniqKey="Zins J" first="James E" last="Zins">James E. Zins</name><affiliation wicri:level="2"><nlm:affiliation>Department of Plastic Surgery, Cleveland Clinic, Cleveland, OH.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>Department of Plastic Surgery, Cleveland Clinic, Cleveland</wicri:cityArea></affiliation></author><author><name sortKey="Schwarz, Graham S" sort="Schwarz, Graham S" uniqKey="Schwarz G" first="Graham S" last="Schwarz">Graham S. Schwarz</name><affiliation wicri:level="2"><nlm:affiliation>Department of Plastic Surgery, Cleveland Clinic, Cleveland, OH.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>Department of Plastic Surgery, Cleveland Clinic, Cleveland</wicri:cityArea></affiliation></author></analytic><series><title level="j">Microsurgery</title><idno type="eISSN">1098-2752</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Anastomosis, Surgical</term><term>Animals</term><term>Axilla</term><term>Feasibility Studies</term><term>Femoral Artery (surgery)</term><term>Femoral Vein (surgery)</term><term>Free Tissue Flaps (blood supply)</term><term>Free Tissue Flaps (transplantation)</term><term>Graft Survival</term><term>Groin (blood supply)</term><term>Groin (surgery)</term><term>Lymph Nodes (blood supply)</term><term>Lymph Nodes (transplantation)</term><term>Lymphedema (surgery)</term><term>Male</term><term>Microsurgery</term><term>Models, Animal</term><term>Rats</term><term>Rats, Inbred Lew</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Aine ()</term><term>Aisselle</term><term>Anastomose chirurgicale</term><term>Animaux</term><term>Artère fémorale ()</term><term>Lambeaux tissulaires libres ()</term><term>Lambeaux tissulaires libres (transplantation)</term><term>Lymphoedème ()</term><term>Microchirurgie</term><term>Modèles animaux</term><term>Mâle</term><term>Noeuds lymphatiques ()</term><term>Noeuds lymphatiques (transplantation)</term><term>Rats</term><term>Rats de lignée LEW</term><term>Survie du greffon</term><term>Veine fémorale ()</term><term>Études de faisabilité</term></keywords><keywords scheme="MESH" qualifier="blood supply" xml:lang="en"><term>Free Tissue Flaps</term><term>Groin</term><term>Lymph Nodes</term></keywords><keywords scheme="MESH" qualifier="surgery" xml:lang="en"><term>Femoral Artery</term><term>Femoral Vein</term><term>Groin</term><term>Lymphedema</term></keywords><keywords scheme="MESH" qualifier="transplantation" xml:lang="en"><term>Free Tissue Flaps</term><term>Lymph Nodes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Anastomosis, Surgical</term><term>Animals</term><term>Axilla</term><term>Feasibility Studies</term><term>Graft Survival</term><term>Male</term><term>Microsurgery</term><term>Models, Animal</term><term>Rats</term><term>Rats, Inbred Lew</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Aine</term><term>Aisselle</term><term>Anastomose chirurgicale</term><term>Animaux</term><term>Artère fémorale</term><term>Lambeaux tissulaires libres</term><term>Lymphoedème</term><term>Microchirurgie</term><term>Modèles animaux</term><term>Mâle</term><term>Noeuds lymphatiques</term><term>Rats</term><term>Rats de lignée LEW</term><term>Survie du greffon</term><term>Veine fémorale</term><term>Études de faisabilité</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Vascularized lymph node transfer (VLNT) is a promising microvascular free flap technique for the surgical treatment of lymphedema. To date, few experimental animal models for VLNT have been described and the viability of lymph nodes after the transfer tested. We aimed to evaluate the feasibility of axillary VLNT in the rat. Lymph node containing flaps were harvested from the axillary region in 10 Lewis rats based on the axillary vessels. Flaps were transferred to the ipsilateral groin and end-to-side microanastomosis was performed to the femoral vessels using 10-0 sutures. Indocyanine green (ICG) angiography was used to confirm flap perfusion. On postoperative day 7, flaps were elevated to assess their structure and vessel patency. Hematoxylin and eosin staining was used to confirm the presence and survival of lymph nodes. All animals tolerated the procedure well. Immediate post-procedure ICG angiography confirmed flap perfusion. No signs of ischemia or necrosis were observed in donor extremities. At postoperative day 7, all flaps remained viable with patent vascular pedicles. Gross examination and histology confirmed the presence of 3.6 ± 0.5 lymph nodes in each flap without any signs of necrosis. This study showed that the transfer of axillary lymph nodes based on the axillary vessels is feasible. The flap can be used without the need for donor animals and it contains a consistent number of lymph nodes. This reliable VLNT can be further utilized in studies involving lymphedema, transplantation, and induction of immunologic tolerance.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Ohio</li></region></list><tree><country name="États-Unis"><region name="Ohio"><name sortKey="Kwiecien, Grzegorz J" sort="Kwiecien, Grzegorz J" uniqKey="Kwiecien G" first="Grzegorz J" last="Kwiecien">Grzegorz J. Kwiecien</name></region><name sortKey="Djohan, Risal" sort="Djohan, Risal" uniqKey="Djohan R" first="Risal" last="Djohan">Risal Djohan</name><name sortKey="Gharb, Bahar Bassiri" sort="Gharb, Bahar Bassiri" uniqKey="Gharb B" first="Bahar Bassiri" last="Gharb">Bahar Bassiri Gharb</name><name sortKey="Korn, Jason" sort="Korn, Jason" uniqKey="Korn J" first="Jason" last="Korn">Jason Korn</name><name sortKey="Madajka, Maria" sort="Madajka, Maria" uniqKey="Madajka M" first="Maria" last="Madajka">Maria Madajka</name><name sortKey="Schwarz, Graham S" sort="Schwarz, Graham S" uniqKey="Schwarz G" first="Graham S" last="Schwarz">Graham S. Schwarz</name><name sortKey="Uygur, Safak" sort="Uygur, Safak" uniqKey="Uygur S" first="Safak" last="Uygur">Safak Uygur</name><name sortKey="Zins, James E" sort="Zins, James E" uniqKey="Zins J" first="James E" last="Zins">James E. Zins</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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